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ATCC ct26 murine crc cells
Ct26 Murine Crc Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3080 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ct26+murine+crc+cells/pm42018579-62-0-4?v=ATCC
Average 99 stars, based on 3080 article reviews

ct26 murine crc cells - by Bioz Stars, 2026-07

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PNE suppresses tumor growth in a CRC syngeneic mouse model. ( A ) Tumor growth curves of CT26-bearing mice treated with PNE. Tumor volume was measured every 3–4 days and calculated using the formula. ( B ) Representative images of excised tumors collected at the end of the study. Scale bar = 10 mm. ( C ) Tumor weights at the experimental endpoint. Statistical differences were analyzed using one-way ANOVA followed by Tukey’s post hoc test. ( D ) Body weight monitoring throughout PNE treatment showing no signs of treatment-related systemic toxicity. ( E ) Histopathological analysis of tumor, liver, and kidney tissues stained with hematoxylin and eosin from control and PNE-treated mice. Scale bar = 100 µm. Data are presented as mean ± SD. * p < 0.05 and ** p < 0.01, compared to the control group.

Journal: Molecules

Article Title: Integrative Mechanistic Investigation of the Anticancer Effects of Panax notoginseng in Colorectal Cancer

doi: 10.3390/molecules31050807

Figure Lengend Snippet: PNE suppresses tumor growth in a CRC syngeneic mouse model. ( A ) Tumor growth curves of CT26-bearing mice treated with PNE. Tumor volume was measured every 3–4 days and calculated using the formula. ( B ) Representative images of excised tumors collected at the end of the study. Scale bar = 10 mm. ( C ) Tumor weights at the experimental endpoint. Statistical differences were analyzed using one-way ANOVA followed by Tukey’s post hoc test. ( D ) Body weight monitoring throughout PNE treatment showing no signs of treatment-related systemic toxicity. ( E ) Histopathological analysis of tumor, liver, and kidney tissues stained with hematoxylin and eosin from control and PNE-treated mice. Scale bar = 100 µm. Data are presented as mean ± SD. * p < 0.05 and ** p < 0.01, compared to the control group.

Article Snippet: Human CRC cells (HCT 116 and HT-29) and murine CRC cells (CT26) were obtained from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Staining, Control

Establishment of a murine colorectal cancer liver metastases model. ( A ) Experimental flowchart for establishing a murine model of colorectal cancer liver metastasis. The day of CT26 inoculation was designated as day 0, on days 5, 7, and 9, two mice were randomly selected for detection of liver metastatic foci. ( B ) On days 5, 7, and 9 post-CT26 inoculation, two mice were randomly selected for detection of liver metastatic foci on H&E-stained sections (black arrows). ( C ) Gross view of liver tumors at death. ( D–F ) H&E sections of liver ( D ) black arrow indicates metastatic foci), lung ( E ) and kidney ( F ) tissues.

Journal: Journal for Immunotherapy of Cancer

Article Title: Vaccinia virus armed with IL-21 can cure murine colorectal cancer liver metastases via intravenous administration

doi: 10.1136/jitc-2025-012479

Figure Lengend Snippet: Establishment of a murine colorectal cancer liver metastases model. ( A ) Experimental flowchart for establishing a murine model of colorectal cancer liver metastasis. The day of CT26 inoculation was designated as day 0, on days 5, 7, and 9, two mice were randomly selected for detection of liver metastatic foci. ( B ) On days 5, 7, and 9 post-CT26 inoculation, two mice were randomly selected for detection of liver metastatic foci on H&E-stained sections (black arrows). ( C ) Gross view of liver tumors at death. ( D–F ) H&E sections of liver ( D ) black arrow indicates metastatic foci), lung ( E ) and kidney ( F ) tissues.

Article Snippet: The murine CRC cell line CT26 was obtained from the American Type Culture Collection and maintained in 1,640 cell culture medium supplemented with 10% fetal bovine serum (FBS).

Techniques: Staining

VVLΔTK-STCΔN1L-mIL21 treatment prolonged the survival of mice with colorectal cancer liver metastases. ( A ) The day of CT26 inoculation was designated as day 0, on days 7, 9, 11, 21, 23, and 25, mice with colorectal cancer liver metastases were treated via tail vein injection with either PBS, 2×10 8 PFU VVLΔTK-STCΔN1L, or VVLΔTK-STCΔN1L-mIL21. The survival time of the mice was subsequently monitored. ( B ) Survival curve of mice with colorectal cancer liver metastases following viral treatment. Kaplan-Meier survival analysis with log-rank (Mantel-Cox) tests was used to assess survival. n=11 mice/group. ( C–D ) H&E stained liver sections from two cured model mice following VVLΔTK-STCΔN1L-mIL21 treatment. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. OV, oncolytic virus; PBS, phosphate-buffered saline; PFU, plaque forming unit.

Journal: Journal for Immunotherapy of Cancer

Article Title: Vaccinia virus armed with IL-21 can cure murine colorectal cancer liver metastases via intravenous administration

doi: 10.1136/jitc-2025-012479

Figure Lengend Snippet: VVLΔTK-STCΔN1L-mIL21 treatment prolonged the survival of mice with colorectal cancer liver metastases. ( A ) The day of CT26 inoculation was designated as day 0, on days 7, 9, 11, 21, 23, and 25, mice with colorectal cancer liver metastases were treated via tail vein injection with either PBS, 2×10 8 PFU VVLΔTK-STCΔN1L, or VVLΔTK-STCΔN1L-mIL21. The survival time of the mice was subsequently monitored. ( B ) Survival curve of mice with colorectal cancer liver metastases following viral treatment. Kaplan-Meier survival analysis with log-rank (Mantel-Cox) tests was used to assess survival. n=11 mice/group. ( C–D ) H&E stained liver sections from two cured model mice following VVLΔTK-STCΔN1L-mIL21 treatment. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. OV, oncolytic virus; PBS, phosphate-buffered saline; PFU, plaque forming unit.

Article Snippet: The murine CRC cell line CT26 was obtained from the American Type Culture Collection and maintained in 1,640 cell culture medium supplemented with 10% fetal bovine serum (FBS).

Techniques: Injection, Staining, Virus, Saline

Viral copy number in murine liver tissue was quantified using RT-qPCR. ( A ) The day of CT26 inoculation was designated as day 0, on days 7, 9 and 11, mice with colorectal cancer liver metastases were treated via tail vein injection with either PBS, 2×10 8 PFU VVLΔTK-STCΔN1L, or VVLΔTK-STCΔN1L-mIL21. On days 14 and 18, the mice were euthanized, and liver tissues were collected for testing. ( B ) DNA was extracted from the liver of mice and the relative viral copies were quantified using RT-qPCR shown as virus copy number per mg tissue DNA. The mean±SEM is shown and a one-way ANOVA with Tukey’s multiple comparison post-test was used to assess significance. n=3–4 mice/group/time point. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. ANOVA, analysis of variance; OV, oncolytic virus; PBS, phosphate-buffered saline; RT-qPCR, reverse transcription quantitative PCR.

Journal: Journal for Immunotherapy of Cancer

Article Title: Vaccinia virus armed with IL-21 can cure murine colorectal cancer liver metastases via intravenous administration

doi: 10.1136/jitc-2025-012479

Figure Lengend Snippet: Viral copy number in murine liver tissue was quantified using RT-qPCR. ( A ) The day of CT26 inoculation was designated as day 0, on days 7, 9 and 11, mice with colorectal cancer liver metastases were treated via tail vein injection with either PBS, 2×10 8 PFU VVLΔTK-STCΔN1L, or VVLΔTK-STCΔN1L-mIL21. On days 14 and 18, the mice were euthanized, and liver tissues were collected for testing. ( B ) DNA was extracted from the liver of mice and the relative viral copies were quantified using RT-qPCR shown as virus copy number per mg tissue DNA. The mean±SEM is shown and a one-way ANOVA with Tukey’s multiple comparison post-test was used to assess significance. n=3–4 mice/group/time point. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. ANOVA, analysis of variance; OV, oncolytic virus; PBS, phosphate-buffered saline; RT-qPCR, reverse transcription quantitative PCR.

Article Snippet: The murine CRC cell line CT26 was obtained from the American Type Culture Collection and maintained in 1,640 cell culture medium supplemented with 10% fetal bovine serum (FBS).

Techniques: Quantitative RT-PCR, Injection, Virus, Comparison, Saline, Reverse Transcription, Real-time Polymerase Chain Reaction

IFN-γ can be detected ex vivo after treatment with VVLΔTK-STCΔN1L-mIL21. ( A–D ) The secretion of IFN-γ from CD45 + cells isolated from the spleens of virus-treated mice after incubation with CT26 cells, KRAS peptide, GP70 peptide and B8R peptide. IFN-γ production in the supernatant was determined using an ELISA. The mean±SEM is shown and a one-way ANOVA with Tukey’s multiple comparison post-test was used to assess significance. (n=3–4 mice/group/time point) *p<0.05,**p<0.01, ***p<0.001,****p<0.0001. ( E–F ) Correlation analysis between IFN-γ (IFNG) expression and the abundance of activated CD8 + T cells and CD8 + T EM cells in COAD samples. The X-axis represents IFN-γ expression levels, while the Y-axis indicates the number of activated CD8 + T cells and CD8 + T EM cells. ( G ) Based on the datasets from the TCGA, the disease-free survival curves of patients with COAD and patients with READ with different IFN-γ expression levels were plotted using GEPIA. ( H ) t-SNE plots of immune cell subsets in 371,223 cells from tumor and adjacent normal tissues of 28 patients with MMR-p and 34 MMR-d colorectal cancer. ( I ) Dot plot showing the percentage of IFN-γ-positive expressing cells and CD8A-positive expressing cells in the corresponding immune cell subsets mentioned above. ANOVA, analysis of variance; COAD, colon adenocarcinoma; IFN, interferon; MMR-d, mismatch repair-deficient; MMR-p, mismatch repair-proficient; PBS, phosphate-buffered saline; READ, rectum adenocarcinoma; TCGA, the cancer genome atlas; T EM , effector memory T cell; t-SNE, t-distributed stochastic neighbor embedding.

Journal: Journal for Immunotherapy of Cancer

Article Title: Vaccinia virus armed with IL-21 can cure murine colorectal cancer liver metastases via intravenous administration

doi: 10.1136/jitc-2025-012479

Figure Lengend Snippet: IFN-γ can be detected ex vivo after treatment with VVLΔTK-STCΔN1L-mIL21. ( A–D ) The secretion of IFN-γ from CD45 + cells isolated from the spleens of virus-treated mice after incubation with CT26 cells, KRAS peptide, GP70 peptide and B8R peptide. IFN-γ production in the supernatant was determined using an ELISA. The mean±SEM is shown and a one-way ANOVA with Tukey’s multiple comparison post-test was used to assess significance. (n=3–4 mice/group/time point) *p<0.05,**p<0.01, ***p<0.001,****p<0.0001. ( E–F ) Correlation analysis between IFN-γ (IFNG) expression and the abundance of activated CD8 + T cells and CD8 + T EM cells in COAD samples. The X-axis represents IFN-γ expression levels, while the Y-axis indicates the number of activated CD8 + T cells and CD8 + T EM cells. ( G ) Based on the datasets from the TCGA, the disease-free survival curves of patients with COAD and patients with READ with different IFN-γ expression levels were plotted using GEPIA. ( H ) t-SNE plots of immune cell subsets in 371,223 cells from tumor and adjacent normal tissues of 28 patients with MMR-p and 34 MMR-d colorectal cancer. ( I ) Dot plot showing the percentage of IFN-γ-positive expressing cells and CD8A-positive expressing cells in the corresponding immune cell subsets mentioned above. ANOVA, analysis of variance; COAD, colon adenocarcinoma; IFN, interferon; MMR-d, mismatch repair-deficient; MMR-p, mismatch repair-proficient; PBS, phosphate-buffered saline; READ, rectum adenocarcinoma; TCGA, the cancer genome atlas; T EM , effector memory T cell; t-SNE, t-distributed stochastic neighbor embedding.

Article Snippet: The murine CRC cell line CT26 was obtained from the American Type Culture Collection and maintained in 1,640 cell culture medium supplemented with 10% fetal bovine serum (FBS).

Techniques: Ex Vivo, Isolation, Virus, Incubation, Enzyme-linked Immunosorbent Assay, Comparison, Expressing, Saline

Effect of Lycium barbarum glycopeptide (LbGP) on CT26 cell proliferation. ( A ) Representative bright-field images of CT26 cells treated with 0–1000 μg/mL LbGP for 72 h (200×; scale bar = 100 μm, Leica DMIL LED inverted microscope, Wetzlar, Germany). ( B ) Quantification of cell proliferation via Trypan Blue assay. ( Left ): Percentage (%) of CT26 viable cells; ( Right ): Total number of CT26 viable cells. ( C ) Quantification of cell proliferation via Cell Counting Kit-8 (CCK-8) assay. n = 3. * p < 0.05; n.s., not significant compared to the control (0 μg/mL).

Journal: International Journal of Molecular Sciences

Article Title: Lycium barbarum Glycopeptide Inhibits Colorectal Cancer Cell Proliferation via Activating p53/p21 Pathway and Inducing Cellular Senescence

doi: 10.3390/ijms26157091

Figure Lengend Snippet: Effect of Lycium barbarum glycopeptide (LbGP) on CT26 cell proliferation. ( A ) Representative bright-field images of CT26 cells treated with 0–1000 μg/mL LbGP for 72 h (200×; scale bar = 100 μm, Leica DMIL LED inverted microscope, Wetzlar, Germany). ( B ) Quantification of cell proliferation via Trypan Blue assay. ( Left ): Percentage (%) of CT26 viable cells; ( Right ): Total number of CT26 viable cells. ( C ) Quantification of cell proliferation via Cell Counting Kit-8 (CCK-8) assay. n = 3. * p < 0.05; n.s., not significant compared to the control (0 μg/mL).

Article Snippet: Murine CT26 CRC cell line (RRID: CVCL_0030) was purchased from American Type Culture Collection (ATCC), Manassas, VA, USA.

Techniques: Glycoproteomics, Inverted Microscopy, Cell Counting, CCK-8 Assay, Control

Effect of LbGP on the clonogenic potential of CT26 cells. ( A ) Representative images and quantification of colony formation in plate assays. ( B ) Representative images and quantification of soft agar colony formation (100×; scale bar = 200 μm, Nikon ECLIPSE Ts2 inverted microscope, Tokyo, Japan). n = 3. * p < 0.05, compared to the control (0 μg/mL).

Journal: International Journal of Molecular Sciences

Article Title: Lycium barbarum Glycopeptide Inhibits Colorectal Cancer Cell Proliferation via Activating p53/p21 Pathway and Inducing Cellular Senescence

doi: 10.3390/ijms26157091

Figure Lengend Snippet: Effect of LbGP on the clonogenic potential of CT26 cells. ( A ) Representative images and quantification of colony formation in plate assays. ( B ) Representative images and quantification of soft agar colony formation (100×; scale bar = 200 μm, Nikon ECLIPSE Ts2 inverted microscope, Tokyo, Japan). n = 3. * p < 0.05, compared to the control (0 μg/mL).

Article Snippet: Murine CT26 CRC cell line (RRID: CVCL_0030) was purchased from American Type Culture Collection (ATCC), Manassas, VA, USA.

Techniques: Inverted Microscopy, Control

Effects of LbGP on p53/p21 expression and cell cycle distribution in CT26 cells. ( A ) Western blot and band intensity quantification of p53 and p21 expression after 72 h of LbGP treatment. Two 0 μg/mL samples were used as technical replicates for both protein blots. GAPDH served as the loading control. ( B ) Flow cytometry analysis of cell cycle distribution at 72 h, showing an increased proportion of cells in S phase and a decreased proportion in G2/M phase. n = 3. * p < 0.05; n.s., not significant compared to the control (0 μg/mL).

Journal: International Journal of Molecular Sciences

Article Title: Lycium barbarum Glycopeptide Inhibits Colorectal Cancer Cell Proliferation via Activating p53/p21 Pathway and Inducing Cellular Senescence

doi: 10.3390/ijms26157091

Figure Lengend Snippet: Effects of LbGP on p53/p21 expression and cell cycle distribution in CT26 cells. ( A ) Western blot and band intensity quantification of p53 and p21 expression after 72 h of LbGP treatment. Two 0 μg/mL samples were used as technical replicates for both protein blots. GAPDH served as the loading control. ( B ) Flow cytometry analysis of cell cycle distribution at 72 h, showing an increased proportion of cells in S phase and a decreased proportion in G2/M phase. n = 3. * p < 0.05; n.s., not significant compared to the control (0 μg/mL).

Article Snippet: Murine CT26 CRC cell line (RRID: CVCL_0030) was purchased from American Type Culture Collection (ATCC), Manassas, VA, USA.

Techniques: Expressing, Western Blot, Control, Flow Cytometry

LbGP induces morphological changes and cellular senescence in CT26 cells. ( A ) Representative bright-field images of CT26 cells after 10 days of LbGP treatment (200×; scale bar = 100 μm, Leica DMIL inverted microscope). White arrowheads indicate elongated and flattened cells. ( B ) Senescence-associated β-galactosidase (SA-β-gal) staining (blue) and quantification in control and LbGP-treated cells following 10-day treatment (200×; scale bar = 100 μm, Nikon ECLIPSE Ts2 inverted microscope). Blue arrowheads indicate SA-β-gal-positive cells. n = 3. * p < 0.05; n.s., not significant compared to the control (0 μg/mL).

Journal: International Journal of Molecular Sciences

Article Title: Lycium barbarum Glycopeptide Inhibits Colorectal Cancer Cell Proliferation via Activating p53/p21 Pathway and Inducing Cellular Senescence

doi: 10.3390/ijms26157091

Figure Lengend Snippet: LbGP induces morphological changes and cellular senescence in CT26 cells. ( A ) Representative bright-field images of CT26 cells after 10 days of LbGP treatment (200×; scale bar = 100 μm, Leica DMIL inverted microscope). White arrowheads indicate elongated and flattened cells. ( B ) Senescence-associated β-galactosidase (SA-β-gal) staining (blue) and quantification in control and LbGP-treated cells following 10-day treatment (200×; scale bar = 100 μm, Nikon ECLIPSE Ts2 inverted microscope). Blue arrowheads indicate SA-β-gal-positive cells. n = 3. * p < 0.05; n.s., not significant compared to the control (0 μg/mL).

Article Snippet: Murine CT26 CRC cell line (RRID: CVCL_0030) was purchased from American Type Culture Collection (ATCC), Manassas, VA, USA.

Techniques: Inverted Microscopy, Staining, Control

LbGP reduces tumor growth in mouse models of CT26 tumor implantation. ( A ) Tumor volume progression over the 21-day administration of LbGP. ( B ) Tumor-free body weight at day 21 post-tumor implantation. ( C ) Representative images of dissected tumors from each treatment group. ( D ) Tumor weight at day 21 post-tumor implantation. n = 8 per group; data represent mean ± SD. * p < 0.05; n.s., not significant compared to phosphate-buffered saline (PBS)-treated mice.

Journal: International Journal of Molecular Sciences

Article Title: Lycium barbarum Glycopeptide Inhibits Colorectal Cancer Cell Proliferation via Activating p53/p21 Pathway and Inducing Cellular Senescence

doi: 10.3390/ijms26157091

Figure Lengend Snippet: LbGP reduces tumor growth in mouse models of CT26 tumor implantation. ( A ) Tumor volume progression over the 21-day administration of LbGP. ( B ) Tumor-free body weight at day 21 post-tumor implantation. ( C ) Representative images of dissected tumors from each treatment group. ( D ) Tumor weight at day 21 post-tumor implantation. n = 8 per group; data represent mean ± SD. * p < 0.05; n.s., not significant compared to phosphate-buffered saline (PBS)-treated mice.

Article Snippet: Murine CT26 CRC cell line (RRID: CVCL_0030) was purchased from American Type Culture Collection (ATCC), Manassas, VA, USA.

Techniques: Tumor Implantation, Saline

Figure 1. Characterization of CRLM derivatives with high potential of liver metastasis. (A) Flowchart of the construction process of CRC cells with high liver metastasis potential. (B) Flowchart of the in vivo selection of liver metastatic derivatives from MC38-GFP-Luc (Parental) and CT26-GFP-Luc (Parental) cells. (C) In vivo bioluminescent imaging (left) and H&E images (right) of corresponding mice for isolating liver metastatic derivatives MC38- LM1, MC38-LM2, MC38-LM3a, and MC38-LM3b at speciﬁed time points. “M” represents liver metastatic foci, while “L” represents adjacent liver tissue. (D) The formation of liver metastatic lesions at speciﬁed endpoints following intrasplenic injection of MC38-P, MC38-LM1, and MC38-LM2 cells. Scale bars, 1 cm. (E) Quantiﬁcation of the number of liver metastasis nodules in (D); one-way ANOVA test. (F) The liver metastasis incidence and endpoints following intrasplenic injection of corresponding cell lines; Chi-Squared Test. (G) Trajectories of cultured MC38-P, MC38-LM3a, and MC38-LM3b were observed over the same duration (6 h), and the cumulative migration distance was quantiﬁed. At least twenty cells were examined and quantiﬁed in each group; one-way ANOVA test. Scale bars, 100 μm. (H) Cell migration and invasion capacities of MC38-P, MC38-LM3a, and MC38-LM3b; one-way ANOVA test.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: RUNX1/SLAMF3 Axis Drives Immunosuppression to Contribute to Colorectal Cancer Liver Metastasis by Blocking Phagocytosis and Depleting C1QC + Tumor-Associated Macrophages.

doi: 10.1002/advs.202506641

Figure Lengend Snippet: Figure 1. Characterization of CRLM derivatives with high potential of liver metastasis. (A) Flowchart of the construction process of CRC cells with high liver metastasis potential. (B) Flowchart of the in vivo selection of liver metastatic derivatives from MC38-GFP-Luc (Parental) and CT26-GFP-Luc (Parental) cells. (C) In vivo bioluminescent imaging (left) and H&E images (right) of corresponding mice for isolating liver metastatic derivatives MC38- LM1, MC38-LM2, MC38-LM3a, and MC38-LM3b at speciﬁed time points. “M” represents liver metastatic foci, while “L” represents adjacent liver tissue. (D) The formation of liver metastatic lesions at speciﬁed endpoints following intrasplenic injection of MC38-P, MC38-LM1, and MC38-LM2 cells. Scale bars, 1 cm. (E) Quantiﬁcation of the number of liver metastasis nodules in (D); one-way ANOVA test. (F) The liver metastasis incidence and endpoints following intrasplenic injection of corresponding cell lines; Chi-Squared Test. (G) Trajectories of cultured MC38-P, MC38-LM3a, and MC38-LM3b were observed over the same duration (6 h), and the cumulative migration distance was quantiﬁed. At least twenty cells were examined and quantiﬁed in each group; one-way ANOVA test. Scale bars, 100 μm. (H) Cell migration and invasion capacities of MC38-P, MC38-LM3a, and MC38-LM3b; one-way ANOVA test.

Article Snippet: Murine CRC cell line CT26 and its liver metastatic derivatives were cultured in RPMI 1640 medium (Procell).

Techniques: In Vivo, Selection, Imaging, Injection, Cell Culture, Migration

Figure 3. Upregulation of SLAMF3 in CRLM derivatives promoted liver metastasis in mice. (A) Volcano plots based on transcriptomic data illustrated the gene expression pattern between MC38-LM3a and MC38-P (left panel), as well as between MC38-LM3b and MC38-P (right panel). (B) Heatmaps based on transcriptomic data illustrated the DEGs between MC38-P and MC38 liver metastatic derivatives. (C-D) Western blot analysis of the expression of SLAMF3 in MC38-P, MC38-LM3a, MC38-LM3b(C), and CT26-P, CT26-LM3 (D). (E) Representative IHC staining for SLAMF3 in liver metastases from the

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: RUNX1/SLAMF3 Axis Drives Immunosuppression to Contribute to Colorectal Cancer Liver Metastasis by Blocking Phagocytosis and Depleting C1QC + Tumor-Associated Macrophages.

doi: 10.1002/advs.202506641

Figure Lengend Snippet: Figure 3. Upregulation of SLAMF3 in CRLM derivatives promoted liver metastasis in mice. (A) Volcano plots based on transcriptomic data illustrated the gene expression pattern between MC38-LM3a and MC38-P (left panel), as well as between MC38-LM3b and MC38-P (right panel). (B) Heatmaps based on transcriptomic data illustrated the DEGs between MC38-P and MC38 liver metastatic derivatives. (C-D) Western blot analysis of the expression of SLAMF3 in MC38-P, MC38-LM3a, MC38-LM3b(C), and CT26-P, CT26-LM3 (D). (E) Representative IHC staining for SLAMF3 in liver metastases from the

Article Snippet: Murine CRC cell line CT26 and its liver metastatic derivatives were cultured in RPMI 1640 medium (Procell).

Techniques: Gene Expression, Western Blot, Expressing, Immunohistochemistry

Figure 5. Mouse CRLM derivatives resisted macrophage phagocytosis and SLAMF3 knockdown enhanced phagocytosis. (A) Representative H&E stain- ing and IHC staining for F4/80 in normal mouse liver and mouse liver metastases. Scale bars, 200 μm. (B) The dots represent the quantiﬁcation of relative F4/80 expression; one-way ANOVA test. (C) Representative IHC staining for CD86 and CD206 in liver metastases. Scale bars, 100 μm. (D) The dots represented quantiﬁcation of relative CD86, CD206 expression; two-way ANOVA test. (E) The correlation analysis between SLAMF3 and tumor- inﬁltrated macrophages and M2 macrophages based on the TCGA database using TIMER2.0. (F) The Flowchart of the in vivo phagocytosis assay process. (G) In vivo phagocytosis assay ﬂow cytometry gating diagram. (H-I) Flow cytometry analysis comparing macrophage-mediated phagocytosis rates between MC38-LM3a (H) or CT26-LM3 (I) and their respective parental cells. (n = 3); unpaired two-tailed Student’s t-test. (J) Flow cytometry anal- ysis of macrophage-mediated phagocytosis rates between MC38-LM3b shScramble and shSLAMF3 (n = 3); one-way ANOVA test. (K) The ﬂowchart of macrophage isolation from mouse CRC liver metastases. (L-N) Western blot and Co-IP analyses were performed on macrophages isolated from mouse CRC liver metastases formed by MC38-P, MC38-LM3b-shScramble, and MC38-LM3b-shSLAMF3.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: RUNX1/SLAMF3 Axis Drives Immunosuppression to Contribute to Colorectal Cancer Liver Metastasis by Blocking Phagocytosis and Depleting C1QC + Tumor-Associated Macrophages.

doi: 10.1002/advs.202506641

Figure Lengend Snippet: Figure 5. Mouse CRLM derivatives resisted macrophage phagocytosis and SLAMF3 knockdown enhanced phagocytosis. (A) Representative H&E stain- ing and IHC staining for F4/80 in normal mouse liver and mouse liver metastases. Scale bars, 200 μm. (B) The dots represent the quantiﬁcation of relative F4/80 expression; one-way ANOVA test. (C) Representative IHC staining for CD86 and CD206 in liver metastases. Scale bars, 100 μm. (D) The dots represented quantiﬁcation of relative CD86, CD206 expression; two-way ANOVA test. (E) The correlation analysis between SLAMF3 and tumor- inﬁltrated macrophages and M2 macrophages based on the TCGA database using TIMER2.0. (F) The Flowchart of the in vivo phagocytosis assay process. (G) In vivo phagocytosis assay ﬂow cytometry gating diagram. (H-I) Flow cytometry analysis comparing macrophage-mediated phagocytosis rates between MC38-LM3a (H) or CT26-LM3 (I) and their respective parental cells. (n = 3); unpaired two-tailed Student’s t-test. (J) Flow cytometry anal- ysis of macrophage-mediated phagocytosis rates between MC38-LM3b shScramble and shSLAMF3 (n = 3); one-way ANOVA test. (K) The ﬂowchart of macrophage isolation from mouse CRC liver metastases. (L-N) Western blot and Co-IP analyses were performed on macrophages isolated from mouse CRC liver metastases formed by MC38-P, MC38-LM3b-shScramble, and MC38-LM3b-shSLAMF3.

Article Snippet: Murine CRC cell line CT26 and its liver metastatic derivatives were cultured in RPMI 1640 medium (Procell).

Techniques: Knockdown, Staining, Immunohistochemistry, Expressing, In Vivo, Phagocytosis Assay, Cytometry, Flow Cytometry, Two Tailed Test, Isolation, Western Blot, Co-Immunoprecipitation Assay

$Cancer cell adhesion during liver colonization and crosstalk to initiate metastasis. αSMA (red)-expressing cells localize in contact with CFSE (green)-labeled CT26 cells (A, B). HSC activation mediated by TaLSECs quantified by αSMA expression (C). Migration of HSCs treated with control medium and LSEC-derived secretome and TaLSEC-derived secretome (D). VEGF secretion by HSCs under control, LSEC secretome stimulation, and TaLSEC secretome stimulation (E). LSEC migration treated with control, LSEC secretome–activated HSCs supernatants, and TaLSEC secretome–activated HSCs supernatants (F). Data were considered statistically significant for * p <0.05 using a one-tailed Mann–Whitney test. Scale bar: 50 µm. Abbreviations: αSMA, α-smooth muscle actin; CFSE, carboxyfluorescein succinimidyl ester; FALS, forward angle light scatter; LFLS, Diffraction light intensity log; LSEC-CM, LSEC conditioned-media; TaLSECs, tumor-activated LSECs.$

Journal: Hepatology Communications

Article Title: Depletion of tumor-reactive HSCs reveals their significance during different stages of liver metastasis

doi: 10.1097/HC9.0000000000000669

Figure Lengend Snippet: Cancer cell adhesion during liver colonization and crosstalk to initiate metastasis. αSMA (red)-expressing cells localize in contact with CFSE (green)-labeled CT26 cells (A, B). HSC activation mediated by TaLSECs quantified by αSMA expression (C). Migration of HSCs treated with control medium and LSEC-derived secretome and TaLSEC-derived secretome (D). VEGF secretion by HSCs under control, LSEC secretome stimulation, and TaLSEC secretome stimulation (E). LSEC migration treated with control, LSEC secretome–activated HSCs supernatants, and TaLSEC secretome–activated HSCs supernatants (F). Data were considered statistically significant for * p <0.05 using a one-tailed Mann–Whitney test. Scale bar: 50 µm. Abbreviations: αSMA, α-smooth muscle actin; CFSE, carboxyfluorescein succinimidyl ester; FALS, forward angle light scatter; LFLS, Diffraction light intensity log; LSEC-CM, LSEC conditioned-media; TaLSECs, tumor-activated LSECs.

Article Snippet: CT26 CRC murine cell line (ATCC), murine CRC cell line MCA38 (Kerafast) were maintained in RPMI-1640, and melanoma cell line B16-F10 (ATCC, LGC Standards SLU) was maintained in DMEM.

Techniques: Expressing, Labeling, Activation Assay, Migration, Control, Derivative Assay, One-tailed Test, MANN-WHITNEY

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